PZO BIOLAR DIC
is a precision research microscope designed for work in transmitted polarized interference light. Its main features include a modular design with a removable interference head, flexible illumination and adaptability to a wide range of analytical methods.
The optical equipment of the BIOLAR microscope can be equipped with achromats with an optical distance of 45 mm. The magnification range of this particular microscope is 125 to 1500×.
The PZO Biolar DIC microscope has been designed as a modular system that offers a wide range of customization options for different applications and analytical methods. Its design includes the following key elements:
modular system: it includes an interchangeable interference head with three birefringent prisms, allowing the use of different techniques (colour field method, differential method and inter-fringe method)
universal condensers: optionally with slit aperture or compensators for detailed illumination matching
high precision: possibility of fine adjustment of the prisms using a micrometer screw with an accuracy of 0.01 mm
lighting flexibility: support for monochromatic light with interference filters (546 nm and 590 nm)
special lenses:10×, 20×, 40× and 100× magnification interference lenses optimised for high resolution
The MPI5 interference head is a key element of the microscope designed for advanced polarization interference techniques. With three integrated birefringent prisms, it offers a wide range of customization options for different observation and analysis methods.
The MPI5 head contains three prisms, each optimized for a specific interference method. The first prism is designed for the differential method, which allows detailed analysis of optical path gradients and fine structures. The second prism is adapted for the strip method, which is ideal for observing fibres, edges and other objects with significant differences in optical properties. The third prism allows for the high-shift colour field method, which is suitable for homogeneous samples such as cells or microorganisms.
Each prism can be quickly replaced by means of a rotating disc, the positions of the prisms being indicated by numbers (1, 2, 3) and the free position "0" allowing light to pass through without interference effect.
Precise control of the prisms is provided by micrometer screws with a precision of 0.01 mm, which allow fine positioning of the prisms both parallel and perpendicular to the optical axis of the microscope. This precision is essential for detailed measurement of the optical path and phase shift. In addition, the top of the head incorporates an integrated analyzer that is rotatable through 360° and adjustable in 45° increments, providing precise control of light polarization.
The slit condeser is one of the basic optical components designed to use techniques based on the interference of polarized light. It features a mechanically adjustable slit consisting of two jaws mounted on sliding elements. These jaws can be independently adjusted by means of controls, allowing the width and position of the slit to be precisely adjusted to the needs of the observation.
The unique design of the condenser also allows the slit to be decentered, which facilitates the creation of an oblique light beam, crucial for some special techniques - oblique. With a maximum jaw spacing of approximately 15 mm, the condenser can also be used for normal brightfield observing.
To ensure optimum quality of the polarized light, a slot for a polarizing filter is integrated in the lower part of the condenser.
The interference condenser with compensators is an advanced optical component of the microscope, designed for qualitative analysis of samples with high light intensity and precision requirements.
The condenser is equipped with a rotating disk that contains four quartz compensators optimized for use with objectives of different magnifications (10×, 20×, 40× and 100×). The individual compensators are marked with their respective numbers, ensuring easy adjustment of the appropriate compensator to the lens being used.
Each compensator can be finely adjusted within ±10°, allowing perfect centering for each lens. Adjustment is made using a special key that is inserted into a designated hole in the condenser body. For normal observation, the rotating disc can be set to the "0" position, which deactivates the compensators and allows observation in bright field or polarized light.
